This real-time RT-PCR assay is a molecular tool designed to detect Foot-and-Mouth Disease viruses belonging to the SAT1/III topotype, which have been circulating in the Middle East and the European Union since late 2025. These viruses are closely related to the BOT/1/77 isolate, which is used as a vaccine strain by some companies. At present, this assay is considered a candidate test and has not yet been fully validated; however, it may be useful for the early detection of this specific topotype.