This document describes the method for the amplification of the gene coding for the

flagelline A. This method is an additional test to the molecular serotyping of Listeria

monocytogenes (MI CEB 13, based on the Doumith et al, 2004 method). This method can be

performed in parallel of the molecular serotyping (MI CEB 13) but is required for the strains

that could be “discordant” (groups IIa and IIc).

Dear Colleagues,

As announced during the last workshop, we dispatch to you the two molecular serotyping EURL Lm in-house methods for molecular serotyping of Listeria monocytogenes (Lm), dealing with:

- the determination of the serogroups (revision);

- the amplification of the flaA gene (new).

A note introducing these 2 methods is also included.