Diagnosis activities

The EURL for Brucellosis provides diagnostic services for the confirmation or negation of disease and for strain characterisation. The EURL tests samples using techniques described in the OIE Manuel of Diagnostic Tests and Vaccines for Terrestrial Aniamals. The front-line diagnostic assays are accredited to ISO/IEC 17025. In addition to testing for Brucellosis, the laboratory provides diagnosis for contagious epididymitis.

 

Bacteriology

All Species Culture of specimens and isolation of Brucella spp except  B. ovis D260
All Species PCR detection of Brucella spp. D261
All Species Culture of specimens, isolation and PCR detection of Brucella spp.  D262
All Species Culture of specimens and isolation of B.ovis D263
All Species Identification and typing of Brucella spp (OIE Manual) D270
All Species SNPs based identification (HRM PCR, sequencing)

 

Serology - Immunology

All Species Serum agglutination in tubes (SAT) I30
All Species Rose bengal test (RBT) I40
All Species Complement fixation test (CFT) I50
Bovine Indirect ELISA for bovine serum I60
Bovine Indirect ELISA for bovine milk I70
Bovine Milk ring test (MRT) I80
Dog Rapid Slide Agglutination test (RSAT) I90
Dog B. canis serum agglutination in tubes (SAT) I91
Dog B. canis Laminar Flow Immuno-Chromatographic Assay I92
Ovine B. ovis Complement Fixation Test (CFT) I100

 

For any request, please contact us at eurl.brucellosis@anses.fr

 

Control activities

Control of reagents and vaccines are performed according to the EU Directive 64/432 and OIE standardisation protocols.

 

Vaccines control

Ruminants Brucella vaccine control (workingseed, complete control) T50
Ruminants Brucella vaccine control (final batch, partial control)  T60

 

Reagents control

All Species SAT Antigen control T80
All Species RBT Antigen control T90
All Species CFT Antigen control T100
Bovine MRT antigen control T110
Bovine Serum iELISA kit control (individual protocol) T120
Bovine Serum iELISA kit control (bulk protocol) T121
Bovine Milk iELISA kit control (bulk protocol) T130
Bovine Initial control of iELISA kit : administrative evaluation T140

 

For any request, please contact us at eurl.brucellosis@anses.fr