This test is a simple spot agglutination test using antigen stained with rose bengal and buffered to a low pH, 3.65 ± 0.05 (Morgan et al., 1969).

MORGAN W.J.B., MACKINNON D.J., LAWSON J.R. & CULLEN G.A. (1969). The rose bengal plate agglutination test in
the diagnosis of brucellosis. Vet. Rec., 85, 636–641.