EURL staphylococci

URL: 
https://eurl-staphylococci.anses.fr
Background type: 
color
Display Name: 
Coagulase Positive Staphylococci
Methods Background: 
Materiau Background: 

22nd meeting of WG 3 on Method validation

Bertrand LOMBARD participated virtually (teleconference) to a part of the last meeting of the Working Group 3 on method validation of the subcommitte ISO/TC 34/SC 9 “Food products-Microbiology”.

It took place on 4-6 December 2018 at Sciensano (BE-NRL for Listeria monocytogenes & coagulase positive staphylococci, Brussels).

WG 3 is in charge of developing the series EN ISO 16140 on method validation/verification in food chain microbiology.

This meeting was mainly dedicated to the preparation of the final vote, further to the ISO/DIS vote and CEN Enquiry, on the 4 following parts:

  • Part 3: method verification;
  • Part 4: method validation in a single laboratory;
  • Part 5: factorial interlaboratory validation for non-proprietary methods;
  • Part 6: validation of alternative (proprietary) methods for microbiological confirmation and typing procedures.

The final votes are expected to be launched by mid-2019. B. Lombard ensured a liaison with WG 2 which provides a statistical expertise to prepare these standards.

Training session of the AT-NRL on ESM for SE detection

On 19 and 20 September 2018, Yacine NIA organised at the NRL a training session at AGES, the Autrian NRL for CPS in Graz. This training session was dedicated to the detection of Staphylococcal Enterotoxins according to the European Screening method. Sigrid Fleck, Kornelia Voetsch, Kathrin Hauser, Andrea Kormann-Klement took part to the practical session.

Technical meeting on confirmatory ELISA with DE-NRL

Yacine NIA from EURL CPS and Kati MIELKE from BfR (DE NRL) organised on 6th September a technical meeting on the confirmatory ELISA for Staphylococcal Enterotoxins in food matrices.

EFSA-ECDC 2017 Annual Report

EFSA and ECDC have just published the European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017: http://www.efsa.europa.eu/en/press/news/181212.

In the chapter on food-borne outbreaks (Chapter 16, p. 209-250), data on FBOs due to bacterial toxins, including staphylococcal enterotoxins, can be found. In addition, a sub-chapter (17.2, p. 251-252) is dedicated to staphylococcal enterotoxins, being a microbiological contaminant covered by a food safety criterion in EC Regulation 2073/2005.

Workshop of the Italian NRL for Official Control Italian Laboratories in Torino (15 November)

Déborah MERDA was invited by the Italian NRL, Istituto Zooprofilattico Sperimentale del Piemonte (IZSTo), to attend its annual workshop for their National official control laboratories (OCL).

The Italian NRL presented different PTs performed along 2018. The OCL presented their recent staphylococcal food poisoning outbreaks and their research project.

The purpose of the attendance of Déborah MERDA was to present the EURL for CPS work on WGS and real time PCR method. These 2 methods aroused enthousiasm among the laboratories, some were even willing to implement them in their laboratory.

 

Training on the detection of staphylococcal enterotoxin genes by multiplex real-time PCR (15-16 November)

Alexandra CAUQUIL & Noémie VINGADASSALON organised a training session held at the EURL for CPS Maisons-Alfort (FR) on 15-16 November.

This training session was dedicated to the detection of se genes in CPS strains by real-time PCR for 3 NLRs (LV, IS & MK).

  • Institute of Food Safety, Animal Health and Environment "BIOR" of Latvia (Lasma EGLITE),
  • Matis ohf of Iceland (Anna Pala VIGNISDOTTIR),
  • and Microbiology of food and feed of Macedonia (Marija RATKOVA).

 

 

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